The Luminescent β-galactosidase Reporter System 3 is a complete system for the analysis of transcriptional regulation using the reporter enzyme β-galactosidase. The kit contains two pLacZ reporter vectors and reagents necessary for 100 chemiluminescent assays to detect enzyme activity. The protocol describes chemiluminescent detection of β-galactosidase via a scintillation counter, tube or plate luminometer, or x-ray film.
Overview
- LacZ vectors for transfection and normalization controls
- Fast, simple colorimetric and chemiluminescent beta-galactosidase assays
- Complete system includes vectors plus the chemiluminescent beta-galactosidase assay
Applications
- Reporter gene assays with the beta-galactosidase reporter
- Monitoring transfection efficiencies & normalizing transfections
- Promoter/enhancer studies
