The LDH Cytotoxicity Detection Kit uses LDH, a stable cytoplasmic enzyme that is present in large amounts in most cells. LDH is released into the cell culture supernatant during cytoplasmic membrane damage and can be easily measured using standard reagents.
Overview
- Safety: No radioactive isotopes required
- Accuracy: Assay results strongly correlate with the number of damaged cells
- High sensitivity: Low cell numbers (e.g., 0.2–2 x 102 cells/well) are easily detected
- Fast: Only 0.5–1 hour required for measurement
- Simple: Relabeling and washing steps not required. Radiation disposal and safety procedures not required
Applications
- Detection of lactate dehydrogenase (LDH, LD) released from damaged cells
- LDH cytotoxicity detection
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