6xHis mAb-HRP Conjugate

Additional information

Brand

Size

100 uL

A monoclonal antibody purified from mouse ascites fluid and conjugated to horseradish peroxidase. The antibody can be used to confirm and/or quantify 6xHis-tagged protein expression by Western blot, ELISA, and immunohistochemistry assays.

Overview

  • 6xhis monoclonal antibody: detects as little as 1 ng of 6xhis-tagged proteins
  • 6xhis monoclonal antibody-HRP conjugate: has high sensitivity and detection does not require a 2° antibody
  • 6xHN polyclonal antibody: detects the 6xHN tag and can be used for Western blotting and ELISA

Applications

Detection via:

  • Western Blot
  • IP
  • ELISA

6xHis Monoclonal Antibody detects 6xHis-LacZ

6xHis Monoclonal Antibody detects 6xHis-LacZ

6xHis Monoclonal Antibody detects 6xHis-LacZ. The protein was purified from lysate on TALON Resin by washing in 10 mM imidazole, and then eluting in 150 mM imidazole buffer. Panel A. IMAC purification fractions of 6xHis-LacZ on TALON Resin were analyzed by SDS-PAGE. Panel B. Albumin-free 6xHis Monoclonal Antibody was used to detect the protein on a Western blot.