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AatII
AatII restriction site:
G | ACGT | C
C | TGCA | G
Supplied buffer: T + BSA
Reaction temperature: 37°C
Effect of DNA methylation: enzyme activity is affected by CG methylase
- Source: Acetobacter aceti
- Genome DNA analysis: Staphylococcus aureus genomic DNA
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may often be cut in presence of DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning
AatII restriction site:
G | ACGT | C
C | TGCA | G
Supplied buffer: T + BSA
Reaction temperature: 37°C
Effect of DNA methylation: enzyme activity is affected by CG methylase
- Source: Acetobacter aceti
- Genome DNA analysis: Staphylococcus aureus genomic DNA
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may often be cut in presence of DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning
