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Next Gen Sequencing products from Clontech, Takara & MN

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RNA-seq solution for low input and poor quality samples

The SMARTer® Universal Low Input RNA Kit for Sequencing produces cDNA from low input amounts of poor quality RNA, using random (N6) primers. The kit works with as little as 2 ng of total RNA, and such samples as FFPE and laser-capture dissection tissues. (Random primed)

Get the application note

Clontech product page

Scientifix product page

Reverse transcribe and amplify cDNA from single cells collected on the Fluidigm C1 system

Fluidigm identified SMARTer cDNA synthesis from Clontech as one of the most robust methods for their single cell mRNA-Seq protocol. This kit has the modified reagent volumes necessary for the Fluidigm system

Clontech product page

Scientifix product page

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RNA-seq solution for single cells

A simple and efficient solution for trancriptome analysis (RNA-seq) from single cells or very low amounts of total RNA (10 pg - 10 ng) that are compatible with Illumina's Genome Analyzer™, HiSeq®, HiScanSQ® or MiSeq® instruments. (dT primed)

Webinar recording: Transcriptome analysis in individual cells using SMART-Seq (SMARTer technology)

Clontech product page

Scientifix product page

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RNA-seq solutions for higher RNA amounts

Clontech's single step SMART process permits unparalleled sensitivity and ensures that full length cDNA is generated and amplified.  Start with 1-2 ng of total RNA. (dT primed)

Learn about SMARTer cDNA synthesis

SMARTer cDNA Synthesis recorded webinar

Clontech product page

Scientifix product page

Prepare high-quality RNA-seq libraries from low input samples

Clontech has a Low Input Library Prep kit that pairs perfectly with the SMARTer Ultra Low Input RNA Kit for Sequencing, and for Illumina Sequencing. Sensitive, fast, robust multiplex library generation for NG Illumina sequencing.

Clontech product page

Scientifix product page

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High fidelity polymerase - Library amplification

Takara's PrimeSTAR™ GXL polymerase is the high-fidelity PCR enzyme of choice for standard reactions as well as more challenging reactions. It tolerates high GC-content, excess template and repeat sequences. PrimeSTAR GXL can extend up to 30 kb on human genomic DNA.

Webinar recording: PCR troubleshooting for high fidelity PCR

High fidelity PCR polymerase overview

Takara product page

Scientifix product page

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Real-time PCR - Library quantification

Takara's expert enzymologists have produced real-time PCR mixes that you can expect a lot from. They provide reproducibility, sensitivity, value, and are compatible with most instruments.  Both the SYBR® detection enzymes and the TaqMan® probe mixes include RNase H in the mix.

qPCR Selection Guide

Real-time PCR Overview

Takara SYBR detection product page

Scientifix SYBR detection product page

Takara Probe detection product page

Scientifix Probe detection product page