ptdTomato-N1 is a mammalian expression vector containing a tandem sequence of the dimeric red fluorescent protein, Tomato. An MCS at the 5′ end of the coding sequence makes it possible to insert a gene of interest in order to express it as an N-terminal fusion with tdTomato. The unmodified vector can be used to express tdTomato in mammalian cells.
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
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